Cationic factors affecting phospholipase activities from human lung
Open Access
- 19 July 1990
- journal article
- research article
- Published by Wiley in British Journal of Pharmacology
- Vol. 100 (3), 447-452
- https://doi.org/10.1111/j.1476-5381.1990.tb15826.x
Abstract
1 The effects of varying H+ and other cation concentrations on phospholipase activity were investigated on two particulate fractions from human lung, corresponding to the mitochondrial and microsomal fractions. 2 Three 14C-labelled substrates, arachidonyl-phosphatidylcholine (PC), -phosphatidylethanolamine (PE) and -phosphatidylinositol (PI) were used. 3 For two substrates, PE and PI, hydrolysis was maximal at pH 6, with either subcellular fraction. 4 Hydrolysis of all three substrates was strongly inhibited by EDTA and EGTA (10–25 mm). Addition of 2,2-dipyridyl, o-phenanthroline, 8-hydroxyquinoline or desferrioxamine (10 μm-1 mm) did not inhibit but often increased hydrolysis of all substrates. 5 Addition of Zn2+, as ZnCl2, (10 μm-1 mm) inhibited PE and PI, but not PC, hydrolysis. 6 The phospholipase activities from human lung appear to be dependent on Ca2+ for maximal activity and to be inhibited by other metal ions including Zn2+.This publication has 29 references indexed in Scilit:
- Phospholipases A2 and C of human lung; subcellular distribution and substrate selectivityBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1989
- Rat platelet arachidonate metabolism in the presence of Ca2+, Sr2+ and Ba2+: studies using intact platelets and semi-purified phospholipase A2Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1987
- Hydrolysis of endogenous phospholipids by rat platelet phospholipase A2: ether or acyl bond and polar head group selectivityBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1987
- Fractionation, biochemical characterization and lysosomal phospholipases of human liverFEBS Letters, 1987
- A cytosolic phospholipase in human neutrophils that hydrolyzes arachidonoyl-containing phosphatidylcholineBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1986
- Phospholipase activity in intact rat glycogen-elicited neutrophilsInflammation Research, 1986
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Lung surfactant synthesis: A Ca++-dependent microsomal phospholipase A2 in the lungBiochemical and Biophysical Research Communications, 1975
- Some properties of phospholipases of alveolar macrophagesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1973
- Some studies on the metabolism of phospholipids in plasma membranes from rat liverBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1971