Spectrum and possible mechanism of carrageenan cytotoxicity.
- 1 August 1971
- journal article
- Vol. 64 (2), 387-404
Abstract
Carrageenan, a sulfated polygalactose which suppresses established delayed hypersensitivity in vivo, is shown to be cytotoxic to macrophages but not to lymphocytes in vitro. This cytotoxicity depends on the carrageenan concentration and degree of lysosomal differentiation but is independent of serum. Survival of macrophages in the presence of carrageenan can be enhanced temporarily by corticosteroids. Ultrastructural studies reveal that carrageenan is readily taken up by macrophages and stored in lysosomes, which subsequently swell and rupture, apparently resulting in cell death. The presence of corticosteroids temporarily retards lysosome swelling. It is suggested that carrageenan may exert its cytotoxic effect by causing osmotic rupture of lysosomes. The possible immunologic significance of these findings is discussed.This publication has 28 references indexed in Scilit:
- Leukotactic Factor Produced by Sensitized LymphocytesScience, 1969
- ULTRASTRUCTURE OF HUMAN LEUKOCYTES AFTER SIMULTANEOUS FIXATION WITH GLUTARALDEHYDE AND OSMIUM TETROXIDE AND "POSTFIXATION" IN URANYL ACETATEThe Journal of cell biology, 1968
- EFFECT OF DMSO ON THE STABILIZATION OF LYSOSOMES BY CORTISONE AND CHLOROQUINE IN VITRO*Annals of the New York Academy of Sciences, 1967
- AN EXAMINATION OF THE CYTOTOXIC EFFECTS OF SILICA ON MACROPHAGESThe Journal of Experimental Medicine, 1966
- Mechanism of a Reaction in Vitro Associated with Delayed-Type HypersensitivityScience, 1966
- THE IN VITRO DIFFERENTIATION OF MONONUCLEAR PHAGOCYTESThe Journal of Experimental Medicine, 1966
- Degraded carrageenan and histamine-induced parietal cell hyperplasia in the guinea-pigJournal of Pharmacy and Pharmacology, 1965
- THE DIFFERENTIATION OF MONONUCLEAR PHAGOCYTESThe Journal of Experimental Medicine, 1965
- THE USE OF SPECIFIC "LYMPHOCYTE" ANTISERA TO INHIBIT HYPERSENSITIVE REACTIONS OF THE "DELAYED" TYPEThe Journal of Experimental Medicine, 1961
- Some protective solutions for tissue-cultured cellsExperimental Cell Research, 1959