Monoclonal antibodies against chromosomal proteins of Drosophila melanogaster
- 1 October 1980
- journal article
- research article
- Published by Springer Nature in Chromosoma
- Vol. 80 (3), 253-275
- https://doi.org/10.1007/bf00292684
Abstract
Total nuclear protein from the embryonic D. melanogaster cell line Kc and crude hydroxyapatite fractions thereof were used for immunization of mice. From the spleen cells of these mice we established 755 permanent lymphoid cell lines using the hybridoma technique originally developed by Köhler and Milstein (1975). Radioimmunoassay showed 455 of these cell lines secreted antibodies which bound to component(s) contained in the antigen mixtures used for immunization. Screening of 311 cell lines using indirect immunofluorescence revealed 58 lines whose antibodies showed a highly selective staining pattern on polytene chromosomes from the salivary glands of D. melanogaster third instar larvae. Eight of these cell lines were cloned and further characterized. We were able to order the staining patterns into three distinct classes based on the staining behaviour of the monoclonal antibodies: staining of active regions, staining of phase dark bands or staining of most interbands. The molecular weight of those antigens against which the monoclonal antibodies were directed was determined in SDS polyacrylamide gels.Keywords
This publication has 44 references indexed in Scilit:
- Immunological identification of a chromocenter-associated protein in polytene chromosomes ofExperimental Cell Research, 1980
- A protein released by DNAase I digestion of drosophila nuclei is preferentially associated with puffsCell, 1978
- Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: Differentiation antigens of rat lymphocytesCell, 1977
- Distribution patterns of three subfractions of drosophila nonhistone chromosomal proteins: possible correlations with gene activityCell, 1977
- Somatic cell hybridization of mouse myeloma cellsCell, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975
- The general affinity of lac repressor for E. coli DNA: Implications for gene regulation in procaryotes and eucaryotesCell, 1975
- Sequential gene activation by ecdysone in polytene chromosomes of Drosophila melanogasterDevelopmental Biology, 1974
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970