A high performance liquid chromatographic method of analysis of 4'-O-tetrahydropyranyladriamycin and their metabolites in biological samples.

Abstract

A method for measuring, 4''-O-tetrahydropyranyladriamycin (THP) and its metabolites in biological samples is described. By reversed-phase high performance liquid chromatography using fluorescence detection, THP and its metabolites were all separated on a single chromatogram within 18 min. A linear calibration curve was obtained up to 2000 ng/ml of THP in plasma. The recovery of THP in the analysis was > 95% above 5 ng/ml and 87.1% even at 1.25 ng/ml. Thus, the lower limit was 1.25ng/ml in biological samples. Blood levels and urinary excretion in mice and dogs were satisfactorily measured by this method.