CHEMICAL, CLINICAL, AND IMMUNOLOGICAL STUDIES ON THE PRODUCTS OF HUMAN PLASMA FRACTIONATION. XXV. THE PREPARATION OF ANTI-A ISOAGGLUTININ REAGENTS FROM MIXED BLOOD OF GROUPS O AND B 12

Abstract

Pooled plasma from group O donors, available in larger amt. than that from group B donors, is superior to the latter in its activity for red cells having the A2-agglutinogen, and is about equal in its activity for A1 red cells. The anti-B activity of group O plasma may be removed. These principles have been applied to the production of anti-A globulin prepns. obtained by the fractionation of human plasma. The resulting reagents, produced from mixtures of O plus B blood, are group-specific, and possess satisfactory agglutinating properties. The methods described are applicable to the large-scale commercial production of blood-grouping reagents.