Nucleotide Sequence of the Restriction Fragment Hind‐F‐EcoRI1 of Simian‐Virus‐40 DNA (Part of the VP1 Gene)

Abstract

The nucleotide sequence of the SV-40 genome region between the cleavage sites for restriction endonucleases EcoRI (map position 0) and HindII (map position 0.05) was determined mainly by a partial chemical DNA degradation procedure. This fragment represents 5.3% of the genome of SV-40 and is located in the late region, internally in the VP1 gene. The message strand shows only 1 open reading frame for translation into protein, which connects to the one for the preceding fragment. On this basis part of the amino acid sequence of the VP1 protein is presented.