Diffusion Across the Nuclear Envelope Inhibited by Depletion of the Nuclear Ca 2+ Store
- 15 December 1995
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 270 (5243), 1835-1838
- https://doi.org/10.1126/science.270.5243.1835
Abstract
Intact, isolated nuclei and a nuclear membrane (ghost) preparation were used to study regulation of the movement of small molecules across the Xenopus laevis oocyte nuclear membrane. In contrast to models of the nuclear pore complex, which assume passive bidirectional diffusion of molecules less than 70 kilodaltons, diffusion of intermediate-sized molecules was regulated by the nuclear envelope calcium stores. After depletion of nuclear store calcium by inositol 1,4,5-trisphosphate or calcium chelators, fluorescent molecules conjugated to 10-kilodalton dextran were unable to enter the nucleus. Dye exclusion after calcium store depletion was not dependent on the nuclear matrix because it occurred in nuclear ghosts lacking nucleoplasm. Smaller molecules and ions (500-dalton Lucifer yellow and manganese) diffused freely into the core of the nuclear ghosts and intact nuclei even after calcium store depletion. Thus, depletion of the nuclear calcium store blocks diffusion of intermediate-sized molecules.Keywords
This publication has 26 references indexed in Scilit:
- Calcium release from the nucleus by InsP3 receptor channelsNeuron, 1995
- Propagation of cytosolic calcium waves into the nuclei of hepatocytesCell Calcium, 1994
- Reconstitution of nuclear protein transport with semi-intact yeast cells.The Journal of cell biology, 1993
- Nuclear export of proteins: The role of nuclear retentionCell, 1993
- The nuclear pore complexFEBS Letters, 1993
- The Nuclear MembraneScience, 1992
- Calcium and calmodulin function in the cell nucleusBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1992
- Interactions and structure of the nuclear pore complex revealed by cryo-electron microscopy.The Journal of cell biology, 1989
- Functional Organization Of The Nuclear EnvelopeAnnual Review of Cell and Developmental Biology, 1988
- Agonists stimulate divalent cation channels in the plasma membrane of human plateletsFEBS Letters, 1985