Human liver RNA-programmed in vitro synthesis of a polypeptide related to human apolipoprotein B
- 30 May 1983
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 156 (1), 63-66
- https://doi.org/10.1016/0014-5793(83)80249-x
Abstract
In an in vitro synthesizing system programmed with RNA from human liver a polypeptide with an estimated M r of 80000 (80 kDa)±1400 (mean±SD, n=5) was synthesized. This polypeptide could be precipitated with antiserum to a narrow density cut of LDL (d=1.030-1.055) or antiserum against the high-M r form of apoB (apoB 100 [4]). The synthesized protein is immunologically related to a 75 kDa protein isolated from LDL. We suggest that the 80 kDa protein represents a primary translation product of apoB synthesized in human liver.Keywords
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