Location of phosphorylation site and DNA‐binding site of a positive regulator, OmpR, involved in activation of the osmoregulatory genes of Escherichia coli
Open Access
- 5 June 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 249 (2), 168-172
- https://doi.org/10.1016/0014-5793(89)80617-9
Abstract
The OmpR protein of Escherichia coli is a positive regulator involved in activation of the ompF and ompC genes which encode the major outer membrane proteins OmpF and OmpC, respectively. By employing recombinant DNA techniques, we isolated the N‐ and C‐terminal halves of the OmpR molecule. From the results of biochemical analyses of these fragments, it was concluded that the N‐terminal portion contains a site involved in phosphorylation by an OmpR‐specific protein kinase EnvZ, whereas the C‐terminal part possesses a DNA‐binding site for the ompC and ompF promoters.Keywords
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