Antibodies against IgAl Protease Are Stimulated Both by Clinical Disease and Asymptomatic Carriage of Serogroup A Neisseria meningitidis

Abstract

IgA1 protease waspurifiedfrom a strain ofserogroup A Neisseria meningitidis subgroup IV-1, representative of bacteria that caused an epidemicof meningococcal meningitis in The Gambia in 1982–1983. ELISAs and immunoblot assays were done using this protease as antigen with paired acute- and convalescent-phase sera from patients from that epidemic and from one in Finland caused by other serogroup A meningococci. Paired sera were also tested from healthy Gambians who were persistent nasopharyngeal carriers, persistent noncarriers, or persons who became carriers after the first serum sample was taken. The results correlated well between the two methods: Antibodies were stimulated by disease or acquisition of carriage, and they remained at a constant levelupon continued carriage.