1. Several glycolipids were isolated from rat kidney and characterized. 2. Minced kidney was dehydrated with acetone and the residue was extracted with chloroform-methanol. The extracts were treated with mild alkali and after removal of the released fatty acids with acetone, the alkali-stable lipids were fractionated on silicic acid, Florisil and DEAE-cellulose columns. 3. The main glycolipids were identified as ceramide monohexoside, cerebroside sulfuric acid ester, and a globoside similar to cytolipin R, while the minor glycolipids were ceramide dihexoside, ceramide trihexoside and a hematoside.