Enzyme Electrode with Collagen-Immobilized Cholesterol Oxidase for the Microdetermination of Free Cholesterol
- 1 January 1979
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 12 (14), 1477-1488
- https://doi.org/10.1080/00032717908082547
Abstract
Cholesterol oxidase was immobilized on collagen films associated to an electrochemical sensor to form a cholesterol electrode. The electrode poised at a potential of + 650 mV vs. Ag/AgCl detects the hydrogen peroxide produced in the enzymatic reaction. This device presents a very high sensitivity and a wide range of linearity (10-7 M-0.8.10-4 M). The use of a non enzymatic electrode associated with the enzymatic one allowed the detection and correction of electrochemical interferences when applied to human sera for free cholesterol determination.This publication has 13 references indexed in Scilit:
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