Localization of a Prostaglandin F2a Receptor in Bovine Corpus luteum Plasma Membranes

Abstract

The distribution of a prostaglandin F_2a receptor in various subcellular fractions from bovine corpora lutea obtained by differential and gradient centrifugation paralleled very closely the distribution in these fractions of 5′-nucleotidase, a marker enzyme for plasma membranes. The fractions most enriched in the receptor and 5′-nucleotidase were relatively free of mitochondria and lysosomes but were contaminated to some extent by elements of the endoplasmic reticulum. From these results it can be concluded that the prostaglandin F_2a receptor is localized on the plasma membranes of the corpus luteum cells. A simple method is described for the purification of plasma membranes from bovine corpora lutea by differential centrifugation.