Oxidation catalyzed by H+ ions improves the silver intensification of 3,3?-diaminobenzidine staining by strongly suppressing tissue argyrophilia
- 1 January 1987
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 87 (6), 615-618
- https://doi.org/10.1007/bf00492479
Abstract
We found that a 15-min treatment of tissue sections with 2 M/l H2O2 dissolved in 6 M/l sulphuric acid strongly suppresses the argyrophilia of tissue elements, thus lacilitating selective silver intensification in histochemical procedures. When applied inserted between the 3,3′-diaminobenzidine reaction and physical development, this treatment allows the selective and sensitive visualization of very low levels of peroxidase activity.This publication has 8 references indexed in Scilit:
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