Mutants of Hansenula polymorpha and Candida boidinii Impaired in their Ability to Grow on Methanol

Abstract

Five mutants of H. polymorpha and C. boidinii were isolated which were impaired in their ability to grow on methanol. Two of these mutants lacked the enzyme triokinase, 1 contained lowered activities of fructose 1,6-bisphosphatase and 2 had unknown lesions. Revertants isolated with wild-type phenotype had regained the missing enzyme activities. The relationship of the mutant phenotypes to the proposed dihydroxyacetone pathway for methanol assimilation is discussed.