General Approach for the Estimation of Octanol/Water Partition Coefficient by Reversed-Phase High-Performance Liquid Chromatography
- 1 June 1984
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 7 (7), 1405-1424
- https://doi.org/10.1080/01483918408074054
Abstract
General approach for the relation of reversed-phase high-performance liquid chromatographic retention behaviour to the hydrophobic properties of drugs was developed. Hydrophobicity of compounds was characterized by the logarithm of 1-octanol/water partition coefficient (log Po/w). Reversed-phase retention times of 26 model compounds (log Po/w values of them ranged from −1.22 to 3.84) were measured using 3 to 5 different mixture of acetonitrile and water as eluent and the logarithm of the capacity ratios (log k′) was calculated. The linear relationship between the log k′ values and the percentage of the acetonitrile in the eluent was tested for each molecule. The slope and the intercept (log k′o) of these straight lines were calculated and introduced to the following regression equation: a, b and c, were constants obtained by least squares method. Correlation coefficient of the equation was 0.95. The quotient of the values of a and b gave the percentage of the acetonitrile in the eluent (48%) by which the chromatographic partition system became the best model of the 1-octanol/water partition system. The proposed method provides the possibility of estimating log Po/w values for compounds which can not be eluted by one isocratic eluent composition.Keywords
This publication has 15 references indexed in Scilit:
- Rm values, retention times and octanol—water partition coefficients of a series of 5-nitroimidazolesJournal of Chromatography A, 1983
- A simplified high-pressure liquid chromatography method for determining lipophilicity for structure-activity relationshipsJournal of Medicinal Chemistry, 1983
- Determination of partition coefficients of pyrido[1,2-a]pyrimidin-4-one derivatives by traditional shake, thin-layer chromatographic and gas—liquid chromatographic methodsJournal of Chromatography A, 1982
- Simple Procedure for Determining Octanol–Aqueous Partition, Distribution, and Ionization Coefficients by Reversed-Phase High-Pressure Liquid ChromatographyJournal of Pharmaceutical Sciences, 1978
- Direct measurement of octanol-water partition coefficients by high-pressure liquid chromatographyJournal of Medicinal Chemistry, 1976
- Liquid-liquid partition coefficients by high-pressure liquid chromatographyJournal of Medicinal Chemistry, 1975
- Determination of partition coefficients by liquid chromatographyJournal of Chromatography A, 1975
- Chromatographic hydrophobic parameters in correlation analysis of structure-activity relationshipsJournal of Chromatography A, 1975
- A Simple Assessment of Partition Data for Correlating Structure and Biological Activity Using Thin-Layer ChromatographyNature, 1965
- p-σ-π Analysis. A Method for the Correlation of Biological Activity and Chemical StructureJournal of the American Chemical Society, 1964