Antibody-Dependent Cellular Cytotoxicity to a Syngeneic Gross Virus-Induced Lymphoma 2

Abstract

Normal W/Fu rat lymphoid cells exerted a specific cytotoxic effect on syngeneic (C58NT)D tumor cells in the presence of sera from W/Fu rats immune to Gross virus-induced lymphoma. The antibody-dependent cellular cytotoxicity (ADCC) reaction did not produce detectable release of nonspecific cytotoxic substances, was not diminished by inhibitors of complement, and was more sensitive in detecting antibodies than a test requiring the external addition of complement. After a single immunization, activity began at day 10 and was maximum about 30–40 days after tumor inoculation. ADCC activity was greatest with peripheral blood Iymphocytes, intermediate with spleen cells, and only slightly positive with lymph node cells. Thymus and bone marrow were always negative. There was no correlation between the capacity to mediate ADCC and the ability to bind to antibody-coated target cell (e.g., form rosettes with antibody-sensitized erythrocytes) in the various normal lymphoid organs.