Assignment of the integration site for simian virus 40 to chromosome 17 in GM54VA, a human cell line transformed by simian virus 40.

Abstract

GM54VA human cells transformed by SV40 were fused with peritoneal macrophages obtained from 3 different mouse strains. All 27 hybrid clones studied were positive for SV40 tumor antigen in 100% of their cells and contained human chromosome 17. Human chromosome 17 was the only human chromosome present in 5 of the hybrid clones. Fusion of GM54VA cells and thymidine kinase (EC 2.7.1.75)-deficient mouse or Chinese hamster fibroblasts resulted in the growth in hypoxanthine-aminopterin-thymidine medium of hybrid clones positive and negative for SV40 tumor antigen. Counterselection of the hybrid clones positive for tumor antigen in medium containing 5-bromo-deoxyuridine resulted in the growth of hybrid cells that were negative for tumor antigen. SV40 is integrated in only 1 of the 2 parental human chromosomes 17. Because the genome of SV40 has been assigned to human chromosome 7 in 2 other SV40-transformed human cell lines, at least 2 different integration sites for SV40 would seem to be present in human cells: 1 located in human chromosome 7 and the other located in human chromosome 17.