Inhibitors of permeability transition interfere with the disruption of the mitochondrial transmembrane potential during apoptosis

Abstract

In a number of experimental systems, the early stage of the apoptotic process, i.e. the stage which precedes nuclear disintegration, is characterized by the breakdown of the inner mitochondrial transmembrane potential (ΔΨ _m). Here we address the question as to whether mitochondrial permeability transition (PT) pores may account for the ΔΨ _m dissipation in lymphocyte apoptosis. Drugs known for their PT‐inhibitory potential (bongkrekic acid, cyclosporin A, and the non‐immunosuppressive cyclosporin A analogue N‐methyl‐Val‐4‐cyclosporin A) are capable of preventing the apoptotic ΔΨ _m disruption. Moreover, pharmacological modulation of PT‐mediated ΔΨ _m dissipation can prevent apoptosis. Thus, while suppressing the ΔΨ _m disruption, bongkrekic acid also inhibits the apoptotic chromatinolysis. In conclusion, these data are compatible with the hypothesis that apoptotic ΔΨ _m disruption is mediated by the formation of PT pores and that PT‐mediated ΔΨ _m disruption is a critical event of the apoptotic cascade.