Comparison of a Whole-Blood Interferon γ Assay With Tuberculin Skin Testing for Detecting Latent Mycobacterium tuberculosis Infection

Abstract

Research from JAMA — Comparison of a Whole-Blood Interferon γ Assay With Tuberculin Skin Testing for Detecting Latent Mycobacterium tuberculosis Infection — ContextIdentifying persons with latent tuberculosis infection (LTBI) is crucial to the goal of TB elimination. A whole-blood interferon γ (IFN-γ) assay, the QuantiFERON-TB test, is a promising in vitro diagnostic test for LTBI that has potential advantages over the tuberculin skin test (TST).ObjectivesTo compare the IFN-γ assay with the TST and to identify factors associated with discordance between the tests.Design and SettingProspective comparison study conducted at 5 university-affiliated sites in the United States between March 1, 1998 and June 30, 1999.ParticipantsA total of 1226 adults (mean age, 39 years) with varying risks of Mycobacterium tuberculosis infection or documented or suspected active TB, all of whom underwent both the IFN-γ assay and the TST.Main Outcome MeasureLevel of agreement between the IFN-γ assay and the TST.ResultsThree hundred ninety participants (31.8%) had a positive TST result and 349 (28.5%) had a positive IFN-γ assay result. Overall agreement between the IFN-γ assay and the TST was 83.1% (κ = 0.60). Multivariate analysis revealed that the odds of having a positive TST result but negative IFN-γ assay result were 7 times higher for BCG-vaccinated persons compared with unvaccinated persons. The IFN-γ assay provided evidence that among unvaccinated persons with a positive TST result but negative IFN-γ assay result, 21.2% were responding to mycobacteria other than M tuberculosis.ConclusionsFor all study participants, as well as for those being screened for LTBI, the IFN-γ assay was comparable with the TST in its ability to detect LTBI, was less affected by BCG vaccination, discriminated responses due to nontuberculous mycobacteria, and avoided variability and subjectivity associated with placing and reading the TST.