Biochemical and genetic properties of site-specific restriction endonucleases in Bacillus globigii
- 1 April 1978
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 134 (1), 338-344
- https://doi.org/10.1128/jb.134.1.338-344.1978
Abstract
B. globigii contains 2 site-specific endonucleases, BglI and BglII. A rapid technique for selection of mutants deficient in each of these enzymes was developed using sensitivity to infection by bacteriophage SP50 as an indication of the levels of enzyme. Mutants defective in BglI, BglII and both BglI and BglII retained the wild-type modification phenotype. Genetic and biochemical studies established that these enzymes are involved in restriction in vivo. Simplified purification procedures for BglI and BglII using these mutants are described.This publication has 19 references indexed in Scilit:
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