The thrombospondin‐like chains of cartilage oligomeric matrix protein are assembled by a five‐stranded α‐helical bundle between residues 20 and 83
- 14 March 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 341 (1), 54-58
- https://doi.org/10.1016/0014-5793(94)80239-4
Abstract
The N-terminal fragment of rat cartilage oligomeric matrix protein (COMP), comprising residues 20–83, was over-expressed in E. coli and purified under non-denaturing conditions. The fragment forms pentamers similar to the assembly domain of the native protein. Its five chains can be covalently linked in vitro by oxidation of cysteines 68 and 71. The fragment adopts a predominantly α-helical structure as judged by circular dichroism spectroscopy. On the basis of these findings we propose the model of a five-stranded α-helical bundle for the assembly domain of COMP. The studied sequence is conserved in thrombospondins 3 and 4 thus raising the possibility that these proteins are also pentamers.Keywords
This publication has 26 references indexed in Scilit:
- Mutations that Allow Disulfide Bond Formation in the Cytoplasm of Escherichia coliScience, 1993
- A Switch Between Two-, Three-, and Four-stranded Coiled Coils in GCN4 Leucine Zipper MutantsScience, 1993
- Isolation and Characterization of the Mouse Thrombospondin 3 (Thbs3) GeneGenomics, 1993
- Identification and characterization of thrombospondin-4, a new member of the thrombospondin gene family.The Journal of cell biology, 1993
- Synthesis of truncated amino-terminal trimers of thrombospondinBiochemistry, 1991
- Predicting Coiled Coils from Protein SequencesScience, 1991
- Variable selection method improves the prediction of protein secondary structure from circular dichroism spectraAnalytical Biochemistry, 1987
- The structure of human thrombospondin, an adhesive glycoprotein with multiple calcium-binding sites and homologies with several different proteins.The Journal of cell biology, 1986
- A critical reappraisal of Waddell's technique for ultraviolet spectrophotometric protein estimationAnalytical Biochemistry, 1983
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970