RNA-protein cross-linking inEscherichia coli30S ribosomal subunits; determination of sites on 16S RNA that are cross-linked to proteins S3, S4, S7, S9, S10, S11, S17, S18 and S21 by treatment withbis-(2-chloroethyl)-methylamine
- 24 April 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (8), 3241-3255
- https://doi.org/10.1093/nar/15.8.3241
Abstract
RNA-protein cross-links were introduced into E. coli 30S ribosomal subunits by treatment with bis-(2-chloroethyl)-methyl-amine. After partial nuclease digestion of the RNA moiety, a number of cross-linked RNA-protein complexes were isolated by a new three-step procedure. Protein and RNA analysis of the individual complexes gave the following results: proteins S4 and S9 are cross-linked to the 16S RNA at positions 413 and 954, respectively. Proteins SII and S21 are both cross-linked to the RNA within an oligonucleotide encompassing positions 693–697, and proteins S17, S10, S3 and S7 are cross-linked within oligonucleo-tides encompassing positions 278–280, 1139–1144, 1155–1158, and 1531–1542, respectively. A cross-link to protein S18 was found by a process of elimination to lie between positions 845 and 851.Keywords
This publication has 13 references indexed in Scilit:
- Refined secondary structure models for the 16S and 23S ribosomal RNA ofEscherichia coliNucleic Acids Research, 1983
- The localization of multiple sites on 16S RNA which are cross-linked to proteins S7 and S8 in Escherichia coli 30S ribosomal subunits by treatment with 2-iminothiolaneNucleic Acids Research, 1983
- Precise localisation of three intra-RNA cross-links in 23S RNA, and aone in 5S RNA, induced by treatment ofEscherichia coli50S ribosomal subunits withbis-(2-chloroethtyl)-methylamineNucleic Acids Research, 1982
- Topography of 16 S RNA in 30 S subunits and 70 S ribosomes accessibility to cobra venom ribonucleaseJournal of Molecular Biology, 1981
- The use of azidoarylimidoesters in RNA-protein cross-linking studies with Escherichia coli ribosomesJournal of Molecular Biology, 1980
- Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli.Proceedings of the National Academy of Sciences, 1978
- Chemical cross-linking of protein to RNA within intact ribosomal subunits from Escherichia coliMolecular Genetics and Genomics, 1978
- Micro thin-layer techniques for rapid sequence analysis of 32P-labeled RNA: Double digestion and pancreatic ribonuclease analysesAnalytical Biochemistry, 1977
- [Isolation of highly purified ribonuclease from cobra (Naja oxiana) venom].1975
- Ribosomal protein neighborhoods. I. S18 and S21 as well as S5 and S8 are neighbors.1972