Isoelectric focusing and isoelectric points of aminoacyl‐tRNA synthetases
- 13 June 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 156 (2), 209-216
- https://doi.org/10.1016/0014-5793(83)80498-0
Abstract
Isoelectric points and isoelectric focusing behaviour of 10 highly purified eukaryotic aminoacyl-tRNA synthetases from 3 sources, Saccharomyces cerevisiae, Euglena gracilis and Phaseolus vulgaris were examined. The pI-values measured on polyacrylamide gels under native conditions are situated between pH 5.0–7.5. A microheterogeneity was observed for 9 enzymes appearing otherwise homogeneous on gel electrophoresis. A compilation of the isoelectric points of aminoacyl-tRNA synthetases is given and literature data are compared with our experimental results.Keywords
This publication has 46 references indexed in Scilit:
- Formation of a catalytically active complex between tRNAAsp and aspartyl-tRNA synthetase from yeast in high concentrations of ammonium sulphateBiochimie, 1982
- Chloroplastic methionyl-tRNA synthetase from wheatBiochemical and Biophysical Research Communications, 1981
- Crystallographic studies on the aspartyl-tRNA synthetase-tRNAAsp system from yeast: The crystalline aminoacyl-tRNA synthetaseJournal of Molecular Biology, 1980
- The use of affinity elution from blue dextran sepharose by yeast tRNA2Val in the complete purification of the cytoplasmic valyl-tRNA synthetase from euglena gracilisBiochemical and Biophysical Research Communications, 1980
- Purification of the chloroplastic valyl-tRNA synthetase from euglena gracilisBiochemical and Biophysical Research Communications, 1979
- Arginyl-tRNA Synthetase from Baker's Yeast. Purification and Some PropertiesEuropean Journal of Biochemistry, 1976
- Complete purification and studies on the structural and kinetic properties of two forms of yeast valyl-tRNA synthetaseBiochimie, 1976
- Occurrence and Particle Character of Aminoacyl‐tRNA Synthetases in the Post‐Microsomal Fraction from Rat LiverEuropean Journal of Biochemistry, 1972
- Crystallisation of trypsin‐modified methionyl‐tRNA synthetase from Escherichia coliFEBS Letters, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970