Reactions of Cytochrome b1 and Nitrate Reductase in a Preparation Solubilized from Escherichia coli*
- 1 May 1963
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 53 (5), 389-397
- https://doi.org/10.1093/oxfordjournals.jbchem.a127713
Abstract
The solubilized nitrate reductase system from E. coli possessed enzyme system(s), by which formate- or vitamin K3-reduced cytochrome b1 was reoxidized by 02, nitrate or chlorate. Reoxidation by O2 was not inhibited by cyanide or azide, but by nitrate or chlorate; it was sensitive to cyanide-and azide-inhibition. Participation of the flavin derivative was proved. Oxidation of cytochrome b1 by nitrate was strongly inhibited by 2-heptyl-4-hydroxyquinoline-N-oxide.Keywords
This publication has 18 references indexed in Scilit:
- Nitrate reductase and related enzymes in Escherichia coliBiochemical Journal, 1960
- Hydroxylamine reductase of a halotolerant micrococcusBiochimica et Biophysica Acta, 1960
- STUDIES ON NITRATE REDUCTASE SYSTEM OF ESCHERICHIA COLI: I. PARTICULATE ELECTRON TRANSPORT SYSTEM. TO NITRATE AND ITS SOLUBILIZATIONThe Journal of Biochemistry, 1959
- Respiration and Light-induced Phosphorylation in Extracts of Rhodospirillum rubrumJournal of Biological Chemistry, 1959
- Inhibition of dihydrocozymase-oxidase activity of heart-muscle preparations of certain cell-free bacterial preparations by 2-heptyl-4-hydroxyquinoline N-oxideBiochemical Journal, 1958
- KINETICS AND INHIBITION OF CYTOCHROME COMPONENTS OF THE SUCCINIC OXIDASE SYSTEM .3. CYTOCHROME-B1958
- FORMIC DEHYDROGENASE AND THE HYDROGENLYASE ENZYME COMPLEX IN COLI-AEROGENES BACTERIAJournal of Bacteriology, 1957
- PYRIDINE NUCLEOTIDE-MENADIONE REDUCTASE FROM ESCHERICHIA COLIJournal of Biological Chemistry, 1954
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951
- On the Effect of Chlorate upon the Nitrate Reduction of Plants I. Experiments with Aspergillus oryzaePhysiologia Plantarum, 1951