The Evaluation Of Cell Nuclei Staining Parameters Regarding The Reproducibility Of Fluorescence Measurements

Abstract

The staining parameters for a quantitative DNA determination of fluorescent cells [chicken erythrocytes] were optimized. Pure dyes and precise control of the staining procedure were applied since reproducibility of measurements is important for a quantitative DNA related analysis of cell population. Chicken erythrocytes and isolated nuclei were tested. The staining procedure with acridine derivatives (acriflavine, proflavine, rivanol) was performed with the fluorescence-Feulgen reaction. The influence of staining parameters, i.e., pH and dye concentration of the staining solution, were evaluated regarding the spectral behavior, the total fluorescence intensity and the reproducibility of results.