RESTORATION OF HEMATOPOIESIS IN DOGS BY INFUSION OF CRYOPRESERVED AUTOLOGOUS PERIPHERAL WHITE CELLS FOLLOWING BUSULFAN-CYCLOPHOSPHAMIDE TREATMENT

Abstract

Canine cryopreserved autologous peripheral buffy coat cells were infused after an otherwise lethal regime of busulfan and cyclophosphamide. In seven dogs so treated, restoration of hematopoiesis occurred within 7 days, and six dogs recovered completely. Comparable colony-forming unit in agar activity was present in freshly processed and cryopreserved buffy coat cells and proved to correlate with in vivo marrow recovery. It was concluded that the peripheral blood is a convenient source for obtaining cells for autologous marrow reconstitution in canines.