Effects of ionic strength and sulfhydryl reagents on the binding of creatine phosphokinase to heart mitochondrial inner membranes
- 1 October 1985
- journal article
- research article
- Published by Springer Nature in Journal of Bioenergetics and Biomembranes
- Vol. 17 (5), 295-303
- https://doi.org/10.1007/bf00751106
Abstract
The concept that creatine phosphokinase is bound to the outer surface of the heart mitochondrial inner membrane originated from observations that the enzyme is retained by water-swollen heart mitochondria and by digitonintreated heart mitochondria suspended in isotonic sucrose. The present study establishes that digitonin-treated mitochondria release creatine phosphokinase in isotonic KCl, and other investigators have reported an identical response for the water-swollen organelles. These observations suggest that mitochondrial creatine phosphokinase is not bound to the outer surface of the inner membrane at a site adjacent to the adenine nucleotide translocase under physiologic conditions.Keywords
This publication has 27 references indexed in Scilit:
- Release of enzymes from adult rat heart myocytes.Circulation Research, 1982
- Control of heart mitochondrial oxygen consumption by creatine kinase: The importance of enzyme localizationBiochemical and Biophysical Research Communications, 1982
- Heart mitochondrial creatine kinase solubilizationArchives of Biochemistry and Biophysics, 1981
- The lack of direct coupling between ATP-ADP translocase and creatine phosphokinase in isolated rabbit heart mitochondriaArchives of Biochemistry and Biophysics, 1980
- Binding of creatine kinase to heart and liver mitochondria in vitroArchives of Biochemistry and Biophysics, 1980
- Mitochondrial creatine kinase. Physical and kinetic properties of the purified enzyme from beef heartBiochemistry, 1979
- Interaction between the creatine kinase of heart mitochondria and oxidative phosphorylationJournal of Molecular and Cellular Cardiology, 1977
- The localization of mitochondrial creatine kinase, and its use for the determination of the sidedness of submitochondrial particlesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1973
- Differential effects of mercurial reagents on membrane thiols and on the permeability of the heart mitochondrionBiochemistry, 1970
- ENZYMATIC PROPERTIES OF THE INNER AND OUTER MEMBRANES OF RAT LIVER MITOCHONDRIAThe Journal of cell biology, 1968