ASSAY OF OXYTOCIN BY RAT MAMMARY GLAND IN VITRO

Abstract

A new method for quantitative assay of oxytocin using strips from rat mammary gland in vitro has been investigated. The method is very suitable for determination of small amounts of oxytocin. Doses down to 2 μ-u. oxytocin have been estimated. The dose-response curve is shown to be linear, and the precision of the method is good (λ =0.10 to 0.14). The effect of adrenaline, noradrenaline, acetylcholine, histamine and 5-hydroxytryptamine on the rat mammary gland has also been tested.