Enhancement in Immunity of Tumor Bearing Mice by Immunization Against Prostaglandin E2

Abstract

C57B1/6 mice hearing a palpable Lewis lung carcinoma (LLC) were immunized against prostaglandin E₂ (PGE₂) in order to prevent the immune suppression typical of tumor bearers. Proliferation in response to concanavalin A (Con A) by normal spleen cells cultured in the presence of PGE₂ or by spleen cells of LLC-bearing mice cultured with Con A alone was suppressed. However, the mitogenic response of spleen cells from PGE₂ immunized LLC-bearing mice was only partially suppressed. The random migration of normal macrophages cultured in the presence of PGE, or of macrophages from LLC-bearing mice cultured in medium alone was enhanced when compared to the random migration of normal macrophages cultured in the absence of PGE₂. In contrast, the random migration of macrophages obtained from PGE₂ immunized LLC-bearing mice was the same as that of normal macrophages cultured in the absence of PGE₂.