Summer and winter distributional patterns of attached diatoms were investigated in Yaquina Bay and estuary, Oregon. Differences in species composition and diversity of diatom assemblages at selected stations from fresh water just below Elk City, Oregon, to the marine waters of lower Yaquina Bay were released to environmental gradients. A total of 16,475 diatoms from 30 samples was separated into 256 species and varieties, of which 97 were found in only one sample, and 72 were represented by a single individual. The most abundant diatoms in the August samples were Fragilaria striatual var. californica, Melosira moniliformis, M. nummuloides, Navicula mutica, and Synedra fasciculata, while in the February samples Achnanthes no.2 and no.4, Navicula diserta, N. mutica, and Nitzschia frustulum var. perpusilla were dominant. Of the most abundant taxa, Navicula no. 2, N. diserta, N. gregaria, Nitzschia frustulum var. perpusilla, Synedra fasciculata and Thalassionema nitzschioides were the most evely distributed among the stations. The mean species diversity for diatom assemblages sampled in February was slightly higher than for assemblages collected in August. In February the mean specific diversity within a genus was higher and the mean generic diversity slightly lower than in August. In general, differences in assemblages were closely related horizontally to the salinity gradient and vertically to the desiccation and insolation gradients. However, biological factors were more important in accounting for differences among assemblages in the summer than in the winter and these factors were primarily species interactions between diatoms and macro—algae.