The chemistry of xanthine oxidase. 9. An improved method of preparing the bovine milk enzyme

Abstract

A relatively simple preparation of bovine milk xanthine oxidase is presented. Yields of 70-90% have been made possible by the use of cysteine to release the enzyme from a particu-late complex. The average specific activity (activity/[image]. ratio) of the product is 100. The purity is 85-95% and indications are given how this may be improved at the expense of yield.