The dynamics of nisine accumulation by its producer Streptococcus lactis, str. MSU was studied. In the course of the producer cultivation on the liquid nutrient medium there was a correlation between total, cellular and filtrate nysine. The content of cellular nysine increased gradually to reach maximum by the 6th day of the strain cultivation. The content of filtrate nisine reached maximum on the 2nd day and declined two-fold by the 7th day of cultivation.