Modulation of Polymorphonuclear Leukocyte-Mediated Antibody-Dependent Cellular Cytotoxicity

Abstract

Human polymorphonuclear leukocytes (PMN) are able to mediate the immunologically specific cytolysis of antibody-coated target cells—antibody-dependent cellular cytotoxicity (ADCC). The initial interaction is divalent cation dependent and is inhibited by ethylenediaminetetraacetic acid (EDTA). Agents capable of elevating adenosine cyclic 3′,5′ monophosphate (cyclic 3′,5′ AMP) and exogenous cyclic 3′,5′ AMP are inhibitory to PMN-mediated ADCC activity, while cholinergic agents capable of elevating guanosine 3′,5′ cyclic monophosphate (cyclic 3′,5′ GMP) and exogenous cyclic 3′,5′ GMP exhibit a facilitory effect. Colchicine in concentrations inhibitory to microtubule formation and pharmacologic agents capable of interfering with granule release are inhibitory to PMN ADCC activity.