Virus Antigens on the Surface of Infected Cells: Binding and Elution of [125I]-labelled Antivirus Antibody

Abstract

We showed recently that the specific binding of [¹²⁵I]-labelled antivaccinia IgG to the surface of vaccinia-infected cells could be used to detect vaccinia antigens and measure antivaccinia antibody (Hayashi, Rosenthal & Notkins, 1972). The present study shows that this technique can be applied to other viruses and that purified antivirus antibody can be obtained by eluting the specifically-bound immunoglobulins from the surface of infected cells.