Bestimmung glykosylierter Hämoglobine mit dem »Fast Hemoglobin Test System«: Methodische Aspekte

Abstract

Tenfold determination of HbA1a+b+c from 10 different blood samples by means of the fast Hb test system gave a standard deviation of 0.12% for the same sample. Serial observations on the same group of subjects without metabolic abnormalities indicated differing quality of various samples in the test system. Measurements on capillary blood, venous blood and heparinized blood gave identical values. Blood or hemolysate could be stored for at least 3 days at 22.5.degree. C, 4.degree. C or -20.degree. C, column eluate at 22.5 .degree. C for at least 5 h without altering the results. Hyperlipemic blood led to the obtaining of falsely high values and had to be washed before analysis. Acute elevation in blood-glucose level induced by an oral glucose tolerance test of maturity-onset diabetics requiring insulin led to a short-lived rise in HbA1a+b+c. The fast Hb test system is, after elimination of certain potential errors, a simple, rapid and reproducible means of measuring HbA1a+b+c. The concentration of HbA1a+b+c apparently mirrors short-lived changes in blood sugar level and not only long-term effects on the metabolic state.