Plasma Membrane Vesicles from Source and Sink Leaves

Abstract

Plasma membrane vesicles (PMVs) were prepared by phase partitioning from microsomal fractions of either sink or source leaves of sugar beet (Beta vulgaris L.). The purity, the internal volume, the sidedness, and the sealingness of PMVs prepared from sink leaves did not differ from those measured with PMVs from source leaves. Yet, in response to an imposed proton motive force, PMVs from source leaves accumulated about 4-fold more sucrose than PMVs from sink leaves. The developmental stage did not affect the uptake of glucose and valine in PMVs prepared from leaf tissues. It was concluded that the sink/source transition is accompanied either by the incorporation into the plasma membrane of leaf cells of proteins mediating proton-sucrose cotransport, or by their activation. N-ethylmaleimide and a polyclonal ascitic fluid directed against the 42-kD region of the plasma membrane containing a putative sucrose carrier inhibited the uptake of sucrose in PMVs from source leaves, but not in PMVs from sink leaves. Sodium dodecyl sulfate gel electrophoresis and western blot suggested that the 42 polypeptide was more abundant in the PMVs from source leaves than in the PMVs from sink leaves.