Effect of chronologic age on induction of cystathionine synthase, uroporphyrinogen I synthase, and glucose-6-phosphate dehydrogenase activities in lymphocytes.
- 1 March 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (3), 1916-1919
- https://doi.org/10.1073/pnas.78.3.1916
Abstract
The activities of cystathionine synthase [L-serine hydro-lyase (adding homocysteine), EC 4.2.1.22], uroporphyrinogen I synthase [porphobilinogen ammonia-lyase (polymerizing), EC 4.3.1.8] and glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate:NADP+ 1-oxidoreductase, EC 1.1.1.49) were measured in phytohemagglutinin-stimulated lymphocytes of young and old human subjects. A significant decrease in activity with age was observed for cystathionine synthase and uroporphyrinogen I synthase but not for glucose-6-phosphate dehydrogenase. These changes could not be related to declining phytohemagglutinin response with aging. Age-related decreases in activity of some enzymes may be relevant for an understanding of the biology of aging. False assignment of heterozygosity and even homozygosity, for certain genetic disorders, such as homocystinuria, may result when low enzyme levels are detected in the lymphocytes of older people.This publication has 16 references indexed in Scilit:
- Monoclonal Antibodies Defining Distinctive Human T Cell Surface AntigensScience, 1979
- Immunological studies of Aging. III. Cytokinetic basis for the impaired response of lymphocytes from aged humans to plant lectins.The Journal of Experimental Medicine, 1977
- The pathogenesis of coronary artery disease. A possible role for methionine metabolism.Journal of Clinical Investigation, 1976
- Impaired Lymphocyte Function in Aged HumansJournal of Clinical Investigation, 1974
- Lymphocyte Phytohemagglutinin Responsiveness, Immunoglobulins and Autoantibodies in Aging HumansThe Journal of Immunology, 1973
- Development of Mammalian Sulfur Metabolism: Absence of Cystathionase in Human Fetal TissuesPediatric Research, 1972
- Cystathionine Synthase Activity in Human Lymphocytes: Induction by PhytohemagglutininJournal of Clinical Investigation, 1972
- Cystathionine Synthase in Tissue Culture Derived from Human Skin: Enzyme Defect in HomocystinuriaScience, 1968
- Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.1968
- Transsulfuration in mammals. Microassays and tissue distributions of three enzymes of the pathway.1965