Clearing-factor lipase in adipose tissue. A medium in which the enzyme activity of tissue from starved rats increases in vitro

Abstract

1. When epididymal fat bodies from starved rats are incubated for 3.5hr. at 37 degrees in a defined medium in vitro the total clearing-factor lipase activity rises to approximately twice its initial value. 2. During the incubation period part of the tissue clearing-factor lipase activity appears in the medium. 3. Heparin, glucose, insulin, and HCO(3) (-) and K(+) ions are shown to be important medium constituents.