A DNA fragment containing the origin of replication of the Escherichia coli chromosome.
- 1 July 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (7), 2720-2724
- https://doi.org/10.1073/pnas.74.7.2720
Abstract
A 38 kilobase [kb] pair region of the E. coli K12 chromosome containing the replication origin was physically mapped with restriction endonucleases EcoRI and HindIII. Replication starts within or very near a 1.3 kb pair HindIII fragment in the middle of this region and proceeds outward in both directions with apparently equal speed. This pattern was observed in dnaA and dnaC temperature-sensitive (ts) initiation mutants at the start of the synchronous round of replication which occurs after downshift from the nonpermissive to the permissive temperature.This publication has 27 references indexed in Scilit:
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