THE EFFECT OF CO2 ON PH IN CULTURE MEDIA FOR ALGAE

Abstract

The pH values of commonly-employed algal media respond markedly to the stream of CO₂-in-air with which such media are frequently bubbled during the culturing of algae. This change in pH can be obviated by: (a) using only 1%CO₂-in-air rather than the unnecessarily higher concentrations often used, and (b) by incorporating 0.05 MSigma 7–9 buffer (tris hydroxymethyl amino-methane) in the medium.