Demonstration of Concanavalin A Receptors on Leptomonas pessoai Cell Membrane*§
- 1 May 1976
- journal article
- Published by Wiley in The Journal of Protozoology
- Vol. 23 (2), 329-333
- https://doi.org/10.1111/j.1550-7408.1976.tb03781.x
Abstract
Leptomonas pessoai promastigotes cultivated in a synthetic medium were agglutinated with concanavalin A (ConA). Agglutination was predominantly of the flagellar-flagellar type, and was inhibited with sucrose or alpha-methyl-D-mannoside. Cell surface polysaccharides and/or glycoproteins were demonstrated by several cytochemical methods at the fine-structural level. A Con A-horseradish peroxidase-diaminobenzidine (DAB) technic was used to detect Con A receptors in the pellicular membrane of the flagellar pocket region and in the flagellar membrane proper. Somewhat less of the Con A-DAB reaction product was observed in the pellicular membrane enclosing the rest of the cell.Keywords
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