The Interaction of Amphotericin B Methyl Ester with Protoplasts of Candida albicans
- 1 September 1976
- journal article
- research article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 96 (1), 117-123
- https://doi.org/10.1099/00221287-96-1-117
Abstract
The interaction of amphotericin B methyl ester (AME) with protoplasts of C. albicans was measured indirectly by following the incorporation of [U-14C]phenylalanine into the acid-indirectly material. The inhibitory effects of AME at the minimum inhibitory concentration were prevented by the addition of 85 mM KCl and 45 mM MgCl2, as shown previously for Saccharomyces cerevisiae. In C. albicans, pretreatment of the yeast before antibiotic addition was unnecessary. KCl and MgCl2 did not prevent AME from binding to the protoplast membrane. This interaction was reversed by incubating the protoplasts in the presence of the protecting salts.This publication has 3 references indexed in Scilit:
- Production of Yeast Cell-wall Lytic Enzymes on a Semi-defined Medium by a StreptomycesNature, 1966
- The Effect of Monovalent Cations on the Inhibition of Yeast Metabolism by NystatinJournal of General Microbiology, 1961
- Enzyme formation in Saccharomyces fragilis. 1. Invertase and raffinaseBiochemical Journal, 1953