Transcription factor AP‐2 activity is modulated by protein kinase A‐mediated phosphorylation
- 5 February 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 444 (1), 27-31
- https://doi.org/10.1016/s0014-5793(99)00021-6
Abstract
We recently reported that APOE promoter activity is stimulated by cAMP, this effect being mediated by factor AP-2 [Garcı́a et al. (1996) J. Neurosci. 16, 7550–7556]. Here, we study whether cAMP-induced phosphorylation modulates the activity of AP-2. Recombinant AP-2 was phosphorylated in vitro by protein kinase A (PKA) at Ser239. Mutation of Ser239 to Ala abolished in vitro phosphorylation of AP-2 by PKA, but not the DNA binding activity of AP-2. Cotransfection studies showed that PKA stimulated the effect of AP-2 on the APOE promoter, but not that of the S239A mutant. Therefore, cAMP may modulate AP-2 activity by PKA-induced phosphorylation of this factor.Keywords
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