The lipids of fish. 3. The acetone-insoluble fraction of an acetone extract of the flesh of the haddock
- 1 July 1953
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 54 (4), 559-569
- https://doi.org/10.1042/bj0540559
Abstract
Some lipids present in a crude acetone extract of haddock flesh are practically insoluble in acetone at O[degree] after purification. This material was fractionated by counter-current distr. between light petroleum and 85% (v/v) ethanol, certain products being further fractionated in absolute ethanol at -30[degree]. 2 fractions consisting mainly of lecithin and 2 of an unidentified lipid were obtained for detailed fatty-acid analyses. The lecithins differed from those isolated previously from the acetone-soluble fractions of the same total tissue extract, containing less C20 and C22, more C18 unsaturated acids and, more total saturated acids. The various groups of unsaturated acids were less highly unsaturated. The fatty acids of the unidentified lipid differed from those of the lecithins mainly in containing more stearic acid than palmitic acid and in respect to unsaturation. The unidentified lipid contained a higher ratio of fatty acids to glycerophosphate than the known glycerophosphatides. The presence in hydrolysates of a substance resembling glycerophos-phoric acid but giving a Ba salt insoluble in water, and of a non-amino base giving a water-insoluble phosphomolybdate, but different from choline, may afford clues to the composition of the unidentified lipid. Minor lipid constituents were identified. The material contained 25% non-lipid impurities, more than the acetone-soluble fraction of the same original extract. Acetone precipitation does not, therefore, always afford purer phospholipid prepns. This non-lipid could not be removed by countercurrent distr. and every fraction was heavily contaminated. Various amino compounds, acid-hydrolyzable P compounds and probably carbohydrates which were found in hydrolysates were possibly of non-lipid origin.Keywords
This publication has 13 references indexed in Scilit:
- The lipids of fish. 1. Content and condition of lipids in the flesh of the haddock (Gadus aeglefinus)Biochemical Journal, 1953
- The lipids of fish. 2. The acetone-soluble lipids of the flesh of the haddockBiochemical Journal, 1953
- The application of counter-current distribution to the separation of phospholipinsBiochemical Journal, 1952
- ISOLATION OF A POLYGLYCEROL PHOSPHATIDE FROM THE ALCOHOL-INSOLUBLE PHOSPHATIDES OF DOG LIVERJournal of Biological Chemistry, 1952
- *UBER DIE ALDEHYDE DER GLYCERINPHOSPHATIDE DES SKELETTMUSKELS UND HERZMUSKELS1952
- STUDIES OF ACETAL PHOSPHOLIPIDES OF BRAIN .3. THE FATTY ALDEHYDES PRESENT IN CRYSTALLINE ACETAL ALPHA-PHOSPHOLIPIDE OF BRAIN1951
- The heterogeneity of the glycerophosphate fraction of rat liverBiochemical Journal, 1950
- Über die Aldehyde der Acetalphosphatide des GehirnsHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1950
- The composition of the liver fats of some New Zealand farm animalsBiochemical Journal, 1937
- On the Purification of PhosphatidesBiochemical Journal, 1912