Quantitative determination of the different higher saturated fatty acids in fat from blood, chyle and faeces, by means of partition chromatography on rubber

Abstract

The extraction, purification and quantitative determination of the saturated fatty acids in blood, chyle and feces are described. In order to carry out the quantitative determination, it is necessary to remove the unsaturated fatty acids by oxidation with KMnO4 in an alkaline medium. The quantitative determination of the saturated fatty acids hexanoic acid (C6), octanoic acid (C8) and decanoic acid (C10) and of the unsaturated [DELTA]13-docosanoic acid (C22) before oxidation with KMnO4, and of the saturated fatty acids dodecanoic acid (C12), tetradecanoic acid (C14), hexadecanoic acid (C16), octadecanoic acid (C18), eicosanoic acid (C20) and docosanoic acid (C22) after oxidation with KMnO4, is carried out by means of reversed-phase partition chromatography according to Boldingh (1950, 1953). Some examples of the application are given.