Effect of Heat and Nucleotides on Human Erythrocyte Inorganic Pyrophosphatase.
- 1 May 1967
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 125 (1), 68-70
- https://doi.org/10.3181/00379727-125-32015
Abstract
Incubation above 37 degrees C caused rapid loss of pyrophos-phatase activity. Cysteine, MgCl2 or pyrophosphate protected the enzyme against irreversible heat inactivation. Ca, Mn and Zn did not protect. Dilution increased the heat lability of the enzyme. Hydrolysis of pyrophosphate was inhibited by nucleotide triphosphates but not by adenosine mono-and diphosphate. Of the nucleotides tested, only inosine triphosphate served as substrate for stroma-free hemolysate?.This publication has 5 references indexed in Scilit:
- CONSTITUTIVE INORGANIC PYROPHOSPHATASE OF ESCHERICHIA COLI .I. PURIFICATION AND CATALYTIC PROPERTIES1966
- CONSTITUTIVE INORGANIC PYROPHOSPHATASE OF ESCHERICHIA COLI .2. NATURE AND BINDING OF ACTIVE SUBSTRATE AND ROLE OF MAGNESIUM1966
- Magnetic Resonance Studies of Metal Activation of Enzymic Reactions of Nucleotides and Other Phosphate Substrates*Biochemistry, 1963
- Adenine nucleotide inhibition of mitochondrial inorganic pyrophosphatase activityBiochimica et Biophysica Acta, 1961
- The role of magnesium in the hydrolysis of sodium pyrophosphate by inorganic pyrophosphataseBiochemical Journal, 1954