A note on the estimation of dehydro-l-ascorbic acid in plant tissues by the Roe & Kuether procedure

Abstract

A modification of the Roe and Kuether (1943) procedure for the estimation of dehydroascorbic acid in plant tissues is described. The dinitrophenylosazone formed from dehydroascorbic acid may be separated from other interfering hydrazones or osazones by chromato-graphy on acid alumina. Evidence of the formation of an open-chain osazone and of a lactone form has been obtained when dehydroascorbic acid reacts with 24-dinitrophenylhydrazine under the conditions of the Roe and Kuether test.