Rapid induction of selective transcription by auxins.
Open Access
- 1 June 1985
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 5 (6), 1197-1203
- https://doi.org/10.1128/mcb.5.6.1197
Abstract
Nuclei isolated from excised soybean plumules that were treated with 2,4-dichlorophenoxyacetic acid (2,4-D) were active in transcription of four auxin-regulated genes or DNA sequences, which have been described previously (G. Hagen, A. Kleinschmidt, and T. Guilfoyle, Planta 162:147-153, 1984). The rates of transcription of the auxin-responsive sequences were 10- to 100-fold greater with nuclei isolated from auxin-treated plumules than with those from untreated plumules. The transcriptional response was also observed with hypocotyls of intact soybean seedlings and hypocotyl sections, as well as with green bean and mung bean plumules that were treated with 2,4-D. Other auxins, including 2,4,5-trichlorophenoxyacetic acid, alpha-naphthaleneacetic acid, and indole-3-acetic acid, also induced the transcriptional response. Increased transcription rates were observed within 5 min after application of auxins to excised plumules, and half-maximal to maximal transcription rates were achieved by 15 min after application of auxins. As little as 10(-7) to 10(-8) M 2,4-D induced a transcriptional response, but maximal transcription rates were achieved at 10(-3) M 2,4-D. Brief treatment with the protein synthesis inhibitor cycloheximide did not inhibit the induction of transcription by auxins. These results clearly demonstrated that auxin-regulated gene expression is under rapid transcriptional control. ImagesThis publication has 19 references indexed in Scilit:
- Control of gene expression by glucocorticoid hormonesBiochemical Journal, 1984
- [35] In Vitro transcription: Whole-cell extractMethods in Enzymology, 1983
- [2] New M13 vectors for cloningMethods in Enzymology, 1983
- Isolation of cloned cDNAs to auxin-responsive poly(A) + RNAs of elongating soybean hypocotylProceedings of the National Academy of Sciences, 1982
- A transcriptionally active, covalently closed minichromosome of cauliflower mosaic virus DNA isolated from infected turnip leavesCell, 1982
- Auxin- and Ethylene-Induced Changes in the Population of Translatable Messenger RNA in Basal Sections and Intact Soybean HypocotylPlant Physiology, 1982
- Early auxin-regulated polyadenylylated mRNA sequences in pea stem tissueProceedings of the National Academy of Sciences, 1982
- Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized filmFEBS Letters, 1977
- Steroid Receptors: Elements for Modulation of Eukaryotic TranscriptionAnnual Review of Biochemistry, 1976
- Isolation and Properties of Nuclei from Control and Auxin-treated Soybean HypocotylPlant Physiology, 1975