Fluorescence Microscopy with Acridine Orange: A Study of Hemopoietic Cells in Fixed Preparations
Open Access
- 31 January 1962
- journal article
- research article
- Published by American Society of Hematology in Blood
- Vol. 19 (2), 200-207
- https://doi.org/10.1182/blood.v19.2.200.200
Abstract
1. A technic for the study of fixed preparations of blood and bone marrow by means of fluorescence microscopy with acridine orange is presented. 2. The distribution of RNA and DNA within the blood cells can be demonstrated cytochemically. 3. The RNA content of the erythroid and myeloid cells decreases with maturation. Mature plasma cells contain large quantities of RNA. 4. It is possible to demonstrate cytoplasmic metachromasia between the AO-RNA complexes of the erythroid, myeloid, and plasmacytic cells.This publication has 3 references indexed in Scilit:
- Supravital Blood Studies, Using Acridine Orange FluorescenceBlood, 1961
- Histochemical differentiation of nucleic acids by means of induced fluorescenceExperimental Cell Research, 1956
- The Endocellular Nucleic Acid Distribution and Plasma Protein Formation in MyelomatosisScandinavian Journal of Clinical and Laboratory Investigation, 1949